Rotavirus neutralizing protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis
نویسندگان
چکیده
منابع مشابه
Genetic and antigenic variation of capsid protein VP7 of serotype G1 human rotavirus isolates.
The deduced amino acid sequences of the outer capsid protein, VP7, of serotype G1 rotavirus clinical isolates collected over a 6 year period (1990-1995) in Melbourne, Australia, were examined. Phylogenetic analysis characterized the sequences into two discrete clusters representing two of the four global lineages of human G1 VP7 proteins. Antigenic characterization using a panel of serotype G1-...
متن کاملStructure of rotavirus outer-layer protein VP7 bound with a neutralizing Fab.
Rotavirus outer-layer protein VP7 is a principal target of protective antibodies. Removal of free calcium ions (Ca2+) dissociates VP7 trimers into monomers, releasing VP7 from the virion, and initiates penetration-inducing conformational changes in the other outer-layer protein, VP4. We report the crystal structure at 3.4 angstrom resolution of VP7 bound with the Fab fragment of a neutralizing ...
متن کاملBacterial expression of the major antigenic regions of porcine rotavirus VP7 induces a neutralizing immune response in mice.
The outer capsid protein of rotavirus, VP7, is a major neutralization antigen. A chimeric protein comprising Escherichia coli (E. coli) outer membrane protein A (OmpA) and part of porcine rotavirus VP7 containing all three antigenic regions (217 amino acids) was expressed in Salmonella and E. coli as an outer-membrane associated protein. Mice immunized intraperitoneally or orally, respectively,...
متن کاملIdentification of two subtypes of serotype 4 human rotavirus by using VP7-specific neutralizing monoclonal antibodies.
Two distinct subtypes of human rotavirus serotype 4 were identified by using neutralizing monoclonal antibodies directed to the major outer capsid glycoprotein, VP7, of strains ST3 (subtype 4A) and VA70 (subtype 4B). Specimens containing serotype 4 rotavirus, obtained from different countries, were examined for subtyping by using solid-phase immune electron microscopy, enzyme-linked immunosorbe...
متن کاملCloning of Rota Virus Outer Capsid Protein (VP7) Gene into the pGEM Vector
Background and Aims: In humans the group A rotaviruses are associated with endemic diarrhea in children under the age of 5, leading to approximately 800,000 deaths every year. Introduction of rotavirus vaccines into childhood immunization programs can contribute to substantial reduction in mortality from rotavirus gastroenteritis in developing countries and virtually eliminating hospitalization...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1985
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.54.3.791-797.1985